pgex 4t1 vector (Addgene inc)
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pgex 4t1 vector
Pgex 4t1 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgex+4t1+vector/pCI-MOR271-1+(Plasmid+%2322376)/pmc12339401-416-71-74
Average 93 stars, based on 2 article reviews
Pgex 4t1 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgex+4t1+vector/pCI-MOR271-1+(Plasmid+%2322376)/pmc12339401-416-71-74
Average 93 stars, based on 2 article reviews
pgex 4t1 vector - by Bioz Stars,
2026-10
93/100 stars
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Purification:Article Title: Reconstitution of BNIP3/NIX-mitophagy initiation reveals hierarchical flexibility of the autophagy machinery Article Snippet: To purify GST-TEX264, the cytosol-exposed domain of TEX264 (28–313 aa) fused to an N-terminal GST-tag was gene-synthesized by GenScript and cloned into a pGEX-4T1 vector ( Addgene_227714 ). .. Proteins were expressed and purified according to the protocol in ref. . To purify GST-FAM134C, the cytosol-exposed domain of FAM134C (250–466 aa) fused to an N-terminal GST-tag was gene-synthesized by GenScript and cloned into a Clone Assay:Article Title: Reconstitution of BNIP3/NIX-mitophagy initiation reveals hierarchical flexibility of the autophagy machinery Article Snippet: To purify GST-TEX264, the cytosol-exposed domain of TEX264 (28–313 aa) fused to an N-terminal GST-tag was gene-synthesized by GenScript and cloned into a pGEX-4T1 vector ( Addgene_227714 ). .. Proteins were expressed and purified according to the protocol in ref. . To purify GST-FAM134C, the cytosol-exposed domain of FAM134C (250–466 aa) fused to an N-terminal GST-tag was gene-synthesized by GenScript and cloned into a Article Title: Reconstitution of BNIP3/NIX-mitophagy initiation reveals hierarchical flexibility of the autophagy machinery Article Snippet: Proteins were expressed and purified according to the protocol in ref. . To purify GFP-tagged NIX-GFP ( Addgene_223736 ), NIX(W36A/L39A)-GFP (ΔLIR) ( Addgene_223748 ), BCL2L13-GFP ( Addgene_223745 ), BCL2L13(W276A/I279A)-GFP (ΔLIR1) ( Addgene_223746 ), BCL2L13(Y213A/I216A)-GFP (ΔLIR2) ( Addgene_223783 ), BCL2L13(I224A/L227A)-GFP (ΔLIR3) ( Addgene_223775 ), BCL2L13(I307A/V310A)-GFP (ΔLIR4) ( Addgene_223776 ), BCL2L13(Y213A/I216A/W276A/I279A)-GFP (ΔLIR1 + 2) ( Addgene_223782 ), BCL2L13(I224A/L227A/W276A/I279A)-GFP (ΔLIR1 + 3) ( Addgene_223780 ), BCL2L13(W276A/I279A/I307A/V310A)-GFP (ΔLIR1 + 4) ( Addgene_223781 ), BCL2L13(I224A/L227A/W276A/I279A/I307A/V310A)-GFP (ΔLIR1 + 3 + 4) ( Addgene_ 223784 ), FUNDC1-GFP ( Addgene_223737 ) and FUNDC1(Y18A/L21A)-GFP (ΔLIR) ( Addgene_223750 ), proteins were expressed and purified according to the protocols in refs. – . .. To purify GST-TEX264, the cytosol-exposed domain of TEX264 (28–313 aa) fused to an N-terminal GST-tag was gene-synthesized by GenScript and cloned into a Article Title: Control of mitophagy initiation and progression by the TBK1 adaptors NAP1 and SINTBAD Article Snippet: .. Linear tetra-ubiquitin fused to GST (GST–4×Ub) was cloned into a Article Title: Control of mitophagy initiation and progression by the TBK1 adaptors NAP1 and SINTBAD. Article Snippet: .. Linear tetra-ubiquitin fused to GST (GST–4×Ub) was cloned into a Plasmid Preparation:Article Title: Reconstitution of BNIP3/NIX-mitophagy initiation reveals hierarchical flexibility of the autophagy machinery Article Snippet: To purify GST-TEX264, the cytosol-exposed domain of TEX264 (28–313 aa) fused to an N-terminal GST-tag was gene-synthesized by GenScript and cloned into a pGEX-4T1 vector ( Addgene_227714 ). .. Proteins were expressed and purified according to the protocol in ref. . To purify GST-FAM134C, the cytosol-exposed domain of FAM134C (250–466 aa) fused to an N-terminal GST-tag was gene-synthesized by GenScript and cloned into a Article Title: Reconstitution of BNIP3/NIX-mitophagy initiation reveals hierarchical flexibility of the autophagy machinery Article Snippet: Proteins were expressed and purified according to the protocol in ref. . To purify GFP-tagged NIX-GFP ( Addgene_223736 ), NIX(W36A/L39A)-GFP (ΔLIR) ( Addgene_223748 ), BCL2L13-GFP ( Addgene_223745 ), BCL2L13(W276A/I279A)-GFP (ΔLIR1) ( Addgene_223746 ), BCL2L13(Y213A/I216A)-GFP (ΔLIR2) ( Addgene_223783 ), BCL2L13(I224A/L227A)-GFP (ΔLIR3) ( Addgene_223775 ), BCL2L13(I307A/V310A)-GFP (ΔLIR4) ( Addgene_223776 ), BCL2L13(Y213A/I216A/W276A/I279A)-GFP (ΔLIR1 + 2) ( Addgene_223782 ), BCL2L13(I224A/L227A/W276A/I279A)-GFP (ΔLIR1 + 3) ( Addgene_223780 ), BCL2L13(W276A/I279A/I307A/V310A)-GFP (ΔLIR1 + 4) ( Addgene_223781 ), BCL2L13(I224A/L227A/W276A/I279A/I307A/V310A)-GFP (ΔLIR1 + 3 + 4) ( Addgene_ 223784 ), FUNDC1-GFP ( Addgene_223737 ) and FUNDC1(Y18A/L21A)-GFP (ΔLIR) ( Addgene_223750 ), proteins were expressed and purified according to the protocols in refs. – . .. To purify GST-TEX264, the cytosol-exposed domain of TEX264 (28–313 aa) fused to an N-terminal GST-tag was gene-synthesized by GenScript and cloned into a Article Title: Reconstitution of BNIP3/NIX-mitophagy initiation reveals hierarchical flexibility of the autophagy machinery Article Snippet: Proteins were expressed and purified according to the protocol in ref. . To purify mCherry–WIPI2d K87A/K88A ( Addgene_223751 ) or mCherry–WIPI2d IDR (364–425 aa) ( Addgene_223790 ), the same expression and purification methods were used as described above for full-length mCherry–WIPI2d, with the exception that for the mCherry–WIPI2d IDR we used the S75 Increase 10/300 column. .. An adapted protocol is available in ref. . To purify GFP-tagged or mCherry-tagged ATG13 IDR, the coding sequence for ATG13 (191–517 aa) or ATG13 (230–517 aa) was fused to an N-terminal 6xHis-TEV-mCherry-tag by cloning into a pET-DUET1 vector ( Addgene_223762 ) or by inserting the coding sequence for ATG13 (191–517 aa), (205–517 aa), (231–517 aa), (191–205_231–517 aa), (191–230 aa), (191–205 aa) or (206–230 aa) into a GST-TEV-EGFP-insert by cloning into a Lysis:Article Title: Control of mitophagy initiation and progression by the TBK1 adaptors NAP1 and SINTBAD Article Snippet: .. Linear tetra-ubiquitin fused to GST (GST–4×Ub) was cloned into a Article Title: Control of mitophagy initiation and progression by the TBK1 adaptors NAP1 and SINTBAD. Article Snippet: .. Linear tetra-ubiquitin fused to GST (GST–4×Ub) was cloned into a Sequencing:Article Title: Reconstitution of BNIP3/NIX-mitophagy initiation reveals hierarchical flexibility of the autophagy machinery Article Snippet: Proteins were expressed and purified according to the protocol in ref. . To purify mCherry–WIPI2d K87A/K88A ( Addgene_223751 ) or mCherry–WIPI2d IDR (364–425 aa) ( Addgene_223790 ), the same expression and purification methods were used as described above for full-length mCherry–WIPI2d, with the exception that for the mCherry–WIPI2d IDR we used the S75 Increase 10/300 column. .. An adapted protocol is available in ref. . To purify GFP-tagged or mCherry-tagged ATG13 IDR, the coding sequence for ATG13 (191–517 aa) or ATG13 (230–517 aa) was fused to an N-terminal 6xHis-TEV-mCherry-tag by cloning into a pET-DUET1 vector ( Addgene_223762 ) or by inserting the coding sequence for ATG13 (191–517 aa), (205–517 aa), (231–517 aa), (191–205_231–517 aa), (191–230 aa), (191–205 aa) or (206–230 aa) into a GST-TEV-EGFP-insert by cloning into a Cloning:Article Title: Reconstitution of BNIP3/NIX-mitophagy initiation reveals hierarchical flexibility of the autophagy machinery Article Snippet: Proteins were expressed and purified according to the protocol in ref. . To purify mCherry–WIPI2d K87A/K88A ( Addgene_223751 ) or mCherry–WIPI2d IDR (364–425 aa) ( Addgene_223790 ), the same expression and purification methods were used as described above for full-length mCherry–WIPI2d, with the exception that for the mCherry–WIPI2d IDR we used the S75 Increase 10/300 column. .. An adapted protocol is available in ref. . To purify GFP-tagged or mCherry-tagged ATG13 IDR, the coding sequence for ATG13 (191–517 aa) or ATG13 (230–517 aa) was fused to an N-terminal 6xHis-TEV-mCherry-tag by cloning into a pET-DUET1 vector ( Addgene_223762 ) or by inserting the coding sequence for ATG13 (191–517 aa), (205–517 aa), (231–517 aa), (191–205_231–517 aa), (191–230 aa), (191–205 aa) or (206–230 aa) into a GST-TEV-EGFP-insert by cloning into a Article Title: Human Immunodeficiency Virus Type 1 Vif Up-Regulates the Expression of Tat via AKT Signaling Pathway: Role of Ubiquitin Specific Protease 17 Article Snippet: Plasmid Myc Vif was made by cloning pNL4-3–derived gene vif in pCMV-Myc plasmid from Clontech, United States, as described earlier ( ). pBlue3′LTR-luc was obtained from NIH AIDS Reference and Reagent Program of NIH, MD, United States. .. Glutathione S-transferase (GST) Tat was generated by cloning pNL4-3 derived tat gene in Generated:Article Title: Human Immunodeficiency Virus Type 1 Vif Up-Regulates the Expression of Tat via AKT Signaling Pathway: Role of Ubiquitin Specific Protease 17 Article Snippet: Plasmid Myc Vif was made by cloning pNL4-3–derived gene vif in pCMV-Myc plasmid from Clontech, United States, as described earlier ( ). pBlue3′LTR-luc was obtained from NIH AIDS Reference and Reagent Program of NIH, MD, United States. .. Glutathione S-transferase (GST) Tat was generated by cloning pNL4-3 derived tat gene in Derivative Assay:Article Title: Human Immunodeficiency Virus Type 1 Vif Up-Regulates the Expression of Tat via AKT Signaling Pathway: Role of Ubiquitin Specific Protease 17 Article Snippet: Plasmid Myc Vif was made by cloning pNL4-3–derived gene vif in pCMV-Myc plasmid from Clontech, United States, as described earlier ( ). pBlue3′LTR-luc was obtained from NIH AIDS Reference and Reagent Program of NIH, MD, United States. .. Glutathione S-transferase (GST) Tat was generated by cloning pNL4-3 derived tat gene in |